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Simple computational technique to quantify nuclear shape asymmetry.

Alexander G Nikonenko1, Yuriy M Bozhok

  • 1Department of Cytology, Bogomoletz Institute of Physiology, Kiev, Ukraine.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|December 20, 2014
PubMed
Summary
This summary is machine-generated.

This study introduces a new method to measure nuclear shape asymmetry in cell images. This technique quantifies asymmetry to aid in nuclear morphometry and diagnostics.

Keywords:
computer-assisted morphometrynuclear shapepoint asymmetryradial asymmetrysoftware

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • The eukaryotic cell nucleus, containing the genome, is enclosed by a membrane.
  • Nuclear shape is regulated by the cytoskeleton, nuclear envelope, and nuclear matrix.
  • Alterations in nuclear shape can indicate cellular pathology.

Purpose of the Study:

  • To introduce a novel technique for quantifying nuclear shape asymmetry in digital images.
  • To address the under-explored symmetry aspects of nuclear shape in research and diagnostics.

Main Methods:

  • Developed a software package to estimate nuclear shape asymmetry from digital images of cell nuclei.
  • Implemented two quantification scenarios: identifying pixels outside the largest inscribed circle and finding pixels without symmetric partners relative to the centroid.
  • Utilized the proportion of asymmetric pixels to estimate nuclear shape asymmetry.

Main Results:

  • The technique successfully quantified nuclear shape asymmetry in digital images.
  • Validation was performed on cell nuclei with distinct shape phenotypes.
  • The proportion of asymmetric pixels served as a reliable measure of shape asymmetry.

Conclusions:

  • Nuclear shape asymmetry is a quantifiable feature with potential diagnostic value.
  • This technique can serve as a valuable addition to nuclear morphometry tools.
  • The method aids in the analysis of cell nuclei for research and pathological assessment.