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RNA editing in plant mitochondria.

P S Covello¹, M W Gray

¹Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.

|October 19, 1989

Summary

RNA editing alters genetic information, changing RNA sequences from their DNA templates. This study details cytidine to uridine conversion in wheat mitochondrial cytochrome c oxidase mRNA, explaining plant mitochondrial coding anomalies.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

The central dogma posits RNA sequences mirror DNA templates.
RNA editing, a process altering RNA post-transcription, challenges this principle.
Known RNA editing types include insertions, deletions, and base conversions.

Purpose of the Study:

To investigate RNA editing in plant mitochondria.
To identify specific RNA editing events in wheat cytochrome c oxidase mRNA.
To explain apparent coding anomalies in plant mitochondrial genomes.

Main Methods:

Analysis of wheat mitochondrial mRNA sequences.
Identification of nucleotide conversions.
Comparison of RNA sequences with their corresponding DNA sequences.

Main Results:

Discovery of cytidine to uridine conversion at multiple sites in wheat mitochondrial cytochrome c oxidase subunit II mRNA.
Demonstration of RNA editing as a mechanism for altering protein-coding sequences in plant mitochondria.
Evidence suggesting RNA editing resolves discrepancies between DNA sequence and protein product.

Conclusions:

RNA editing is a significant phenomenon in plant mitochondrial gene expression.
The observed editing reconciles coding anomalies in wheat mitochondria.
This finding broadens the understanding of post-transcriptional gene regulation in eukaryotes.

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