Related Experiment Video
Updated: Apr 19, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Complete Genome Sequence for the Shellfish Pathogen Vibrio coralliilyticus RE98 Isolated from a Shellfish Hatchery
Gary P Richards1, James L Bono2, Michael A Watson3
1U.S. Department of Agriculture, Agricultural Research Service, Dover, Delaware, USA gary.richards@ars.usda.gov.
Whole genome sequencing identified Vibrio coralliilyticus strain RE98, a coral and shellfish pathogen, previously misclassified. This study details its complete genome, comprising two chromosomes and two megaplasmids.
Area of Science:
- Marine microbiology
- Genomics
- Pathogen identification
Background:
- Vibrio coralliilyticus is a known pathogen affecting marine ecosystems, specifically corals and larval shellfish.
- Accurate identification of bacterial pathogens is crucial for effective disease management in aquaculture and conservation efforts.
Purpose of the Study:
- To definitively identify the taxonomic classification of Vibrio strain RE98 using whole genome sequencing.
- To characterize the genomic structure of Vibrio strain RE98.
Main Methods:
- Whole genome sequencing of Vibrio strain RE98.
- Bioinformatic analysis of the resulting sequence data to determine genomic composition.
Main Results:
- Whole genome sequencing confirmed that Vibrio strain RE98 is Vibrio coralliilyticus, not Vibrio tubiashii as previously suggested.
- The genome of V. coralliilyticus RE98 consists of 6,037,824 base pairs.
- The genome is organized into two chromosomes (3,420,228 bp and 1,917,482 bp) and two megaplasmids (380,714 bp and 319,400 bp).
Conclusions:
- Strain RE98 is confirmed as Vibrio coralliilyticus, clarifying its identity.
- The detailed genomic information provides a foundation for future research into V. coralliilyticus virulence and host interactions.
Related Concept Videos
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Bacterial Phylum Verrucomicrobiota
DNA Bacteriophages
Stringent Response in E. coli
Bacterial Phylum Tenericutes
Bacterial Phylum Proteobacteria

