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Transcriptional trans-repression by the c-myb proto-oncogene product
H Nakagoshi1, T Nagase, Y Ueno
1Laboratory of Molecular Genetics, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Nucleic Acids Research
|September 25, 1989
Abstract:
We report that the c-myb protein binds to another site, MBS-II, in the SV40 enhancer with low affinity. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of the sequence containing the MBS-II site induced c-myb-dependent transcriptional repression. Results of mutational analyses of the sequence around the MBS-II site suggested that the c-myb protein represses transcription by competing with another trans-activator. These results indicate that c-myb protein can regulate transcription not only positively but also negatively.