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Updated: Apr 19, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
Published on: October 18, 2022
Multistage regulator based on tandem promoters and CRISPR/Cas
Hangxing Jia1, Tong Liang, Zhaoning Wang
1College of Life Sciences, Wuhan University , Wuhan, Hubei 430072, China.
Abstract:
Accurately controlling expression of target genes between several designed levels is essential for low-noise gene network and dynamic range of gene expression. However, such manipulations have been hard to achieve due to technical limitations. Based on tandem promoters and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, we constructed a multistage regulator that could stably regulate the expression of the reporter gene on three levels, with more than 2-fold difference between each of them. Our findings provide novel insights into constructing a more powerful gene regulation system.
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