Related Experiment Video

Updated: Apr 19, 2026

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
06:01

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Published on: July 3, 2020

9.8K

Spatial control of calcineurin in response to heat shock in fission yeast

Mari Higa1, Ayako Kita, Kanako Hagihara

  • 1Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka, Osaka, 577-8502, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|December 23, 2014
PubMed

Insights

Heat shock triggers calcineurin

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Stress Response

Background:

  • Calcineurin (PPb1) is a Ca2+/calmodulin-dependent protein phosphatase regulating key cellular processes in fission yeast.
  • Calcineurin dephosphorylates transcription factor Prz1, promoting its nuclear translocation and CDRE-dependent gene expression.
  • The spatial regulation of calcineurin under stress conditions remains largely uncharacterized.

Purpose of the Study:

  • To investigate the spatial regulation of calcineurin in response to heat shock in fission yeast.
  • To elucidate the role of calcineurin's phosphatase activity in its stress-induced localization.
  • To explore the connection between calcineurin and RNA granules during heat stress.

Main Methods:

  • Microscopy to observe subcellular localization of calcineurin.
  • Analysis of Prz1 nuclear translocation and CDRE-dependent gene expression.
  • Investigating calcineurin localization in the presence of inhibitors (FK506) and constitutively active forms.
  • Depletion studies of calcineurin and observation of RNA granule formation.

Main Results:

  • Heat shock induces calcineurin-dependent nuclear translocation of Prz1 and CDRE gene expression.
  • Calcineurin relocalizes from diffuse cytoplasmic distribution to dot-like structures (RNA granules) upon heat shock.
  • Calcineurin's phosphatase activity is crucial for its granule localization.
  • Calcineurin depletion leads to the rapid formation of RNA granules.

Conclusions:

  • Calcineurin dynamically regulates gene expression in response to heat shock by altering its subcellular localization.
  • Calcineurin is a component of RNA granules during heat stress, and its phosphatase activity is essential for this localization.
  • These findings reveal a novel role for calcineurin in cellular stress response pathways involving RNA granules.

More Related Videos

Measurements of Physiological Stress Responses in C. Elegans10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

15.3K
Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging06:09

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Published on: December 2, 2016

7.5K

Related Concept Videos

Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
560
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
682
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
940
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
15.9K
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
494