Generation and application of polyclonal IgY antibodies specific for full-length and nicked prostate-specific antigen
Agnieszka Łupicka-Słowik1, Maciej Walczak, Renata Grzywa
1Division of Medicinal Chemistry & Microbiology, Faculty of Chemistry, Wroclaw University of Technology, Wroclaw, Poland.
Background:
The prostate-specific antigen (PSA) is considered an important serum marker for prostate cancer detection, monitoring and staging. The purpose of this study was to generate IgY class antibodies that recognize native PSA and selected epitopes.
Methodology:
Hens immunized with either full-length human PSA or its peptidyl fragment-conjugates produced specific antibodies that were isolated from egg yolks. We developed a monoclonal/IgY sandwich ELISA with a PSA detection limit of 50 pg/ml and a linear range of 0.05-1.0 ng/ml.
Conclusion:
Because the signal observed for the PSA-specific IgY antibodies by ELISA and the reactivity profile of the epitope-derived IgYs were comparable to those of mouse monoclonal IgG antibodies, avian antibodies may be a cost-effective alternative to mammalian antibodies for prostate cancer diagnostics.
