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Competitive peptide antagonists of ANF-induced cyclic guanosine monophosphate production
T J Abell1, A M Richards, T G Yandle
1Department of Medicine, Princess Margaret Hospital, Christchurch, New Zealand.
Biochemical and Biophysical Research Communications
|October 16, 1989
Abstract:
Atrial natriuretic factor (isoleucine ANF 101-126), cleaved ANF (isoleucine ANF 101-105/106-126) and des (Gln 18, Ser 19, Gly 20, Leu 21, Gly 22) ANF 4-23-NH2 (C-ANF 4-23) stimulated cyclic guanosine monophosphate production (cGMP) by rat aortic vascular smooth muscle cells (VSMC) in culture. Cleaved ANF and ANF C4-23 also antagonised or diminished the response to ANF 101-126. Agonist and antagonist actions of both peptides were dose-related. In contrast, prepro ANF (104-123), an ANF precursor fragment, exhibited no agonist or antagonist effect on cGMP production.