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Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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Double Resonance Techniques: Overview01:12

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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Positron Emission Tomography01:29

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Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
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Proton (¹H) NMR: Chemical Shift01:07

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Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
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¹H NMR: Complex Splitting01:13

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A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
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Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
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Proton-Electron Double-Resonance Imaging of pH using phosphonated trityl probe.

Wataru Takahashi1, Andrey A Bobko2, Ilirian Dhimitruka2

  • 1Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Internal Medicine and Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA ; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan.

Applied Magnetic Resonance
|December 23, 2014
PubMed
Summary

Variable Radio Frequency Proton-Electron Double-Resonance Imaging (VRF PEDRI) offers high-resolution pH mapping of aqueous samples using a novel pH-sensitive probe. This technique allows for rapid and accurate pH measurements with potential for in vivo applications.

Keywords:
Overhauser-enhanced magnetic resonance imagingPH mappingProton-Electron Double-Resonance ImagingTrityl radical

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Area of Science:

  • Magnetic Resonance Imaging
  • Biophysical Chemistry
  • Spectroscopy

Background:

  • Variable Radio Frequency Proton-Electron Double-Resonance Imaging (VRF PEDRI) is an advanced technique for functional imaging.
  • Paramagnetic probes are crucial for enhancing signal and enabling specific measurements.
  • Existing methods may have limitations in speed, resolution, or applicability for pH mapping.

Purpose of the Study:

  • To evaluate the potential of VRF PEDRI for precise pH mapping of aqueous samples.
  • To utilize a newly synthesized pH-sensitive phosphonated trityl radical (pTR) probe for this application.
  • To demonstrate the feasibility of low-power, rapid pH mapping.

Main Methods:

  • Employing VRF PEDRI with a pH-sensitive pTR probe.
  • Acquiring images at pre-selected EPR frequencies corresponding to protonated and deprotonated probe states.
  • Calculating pH maps based on the ratio of Overhauser enhancements at different frequencies.

Main Results:

  • Successful extraction of pH maps from aqueous samples using VRF PEDRI and pTR.
  • Demonstrated high spatial resolution (approx. 1 mm) and short acquisition times (130 s).
  • Achieved pH mapping at low Electron Paramagnetic Resonance (EPR) irradiation power (1.25 W), minimizing sample overheating.

Conclusions:

  • VRF PEDRI is a viable and effective method for high-resolution pH mapping.
  • The pTR probe exhibits suitable properties for sensitive and accurate pH measurements.
  • The low-power requirements make VRF PEDRI with pTR promising for in vivo biological studies.