Related Experiment Video
Updated: Apr 19, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Relationship between certain T helper cytokines and ANA staining: who is the helper?
Jinhui Hu1, Yuyan Zhang2, Xinghui Liu1
1Department of Laboratory Medicine, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai 200135, China.
Objective:
This study investigated some T helper cytokines' association with different ANA positive sera.
Methods:
Sera of 53 ANA-positive subjects (11 were stained of homogeneous pattern, 12 of speckled particle pattern, 9 of nucleolar pattern, 15 of centromere pattern, and 6 of the peripheral nuclear type) were collected and analyzed by ELISA for related cytokines and concentrations were compared with each other and 16 healthy donors.
Results:
Investigated cytokines including IL-12(p70) and IFN-γ varied differently in the ANA sera.
Conclusion:
This study demonstrated that some T helper cytokines varied with different ANA-positive sera, suggesting that they may play different roles in related autoimmune diseases.
More Related Videos
06:37Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety
Published on: September 15, 2023
06:15Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
Published on: September 7, 2018
Related Concept Videos
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...