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Updated: Apr 19, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR
Jesse J Waggoner1, Nikita S D Sahadeo2, Arianne Brown3
1Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA, USA.
Abstract:
Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).

