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[3H]WEB 2086 labels platelet activating factor receptors in guinea pig and human lung
G Dent1, D Ukena, G W Sybrecht
1Department of Thoracic Medicine, National Heart and Lung Institute, London, U.K.
European Journal of Pharmacology
|October 10, 1989
Abstract:
The radiolabelled platelet activating factor (PAF) receptor antagonist, [3H]WEB 2086, bound to specific sites on membrane fractions from homogenised guinea pig and human lungs. The sites on guinea pig and human membranes bound [3H]WEB 2086 with dissociation constants (KD) of 16.8 and 22.6 nM and binding capacities (Bmax) of 203 and 157 fmol/mg protein, respectively. In both species, binding was displaced competitively by PAF, suggesting that the sites labelled by [3H]WEB 2086 are PAF receptors.