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Obtaining from grape pomace an enzymatic extract with anti-inflammatory properties
B Rodríguez-Morgado1, M Candiracci, C Santa-María
1Departamento de Bioquímica y Biología Molecular, Universidad de Sevillla, C/Profesor García González, 2, 41012, Sevilla, Spain.
Plant Foods for Human Nutrition (Dordrecht, Netherlands)
|December 24, 2014
Summary
Grape pomace extract (GP-EE) shows anti-inflammatory properties by reducing inflammatory markers in microglia cells. This natural extract may benefit neurodegenerative diseases involving neuroinflammation.
Area of Science:
- Food Science
- Biochemistry
- Pharmacology
Background:
- Grape pomace is a winemaking by-product abundant in bioactive compounds.
- An enzymatic process yields a water-soluble extract (GP-EE) rich in polyphenols, peptides, carbohydrates, and lipids.
- Polyphenols constitute 12% of GP-EE, with flavonoids and phenolic acids being major components.
Purpose of the Study:
- To evaluate the in vitro anti-inflammatory potential of GP-EE.
- To assess GP-EE's effect on inflammatory molecule expression in lipopolysaccharide (LPS)-stimulated N13 microglia cells.
Main Methods:
- Enzymatic extraction of grape pomace to obtain GP-EE.
- Stimulation of N13 microglia cells with LPS.
- Monitoring mRNA levels of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), ionized calcium binding adaptor molecule-1 (Iba-1), and Toll-like receptor-4 (TLR-4).
- Assessing iNOS protein levels in LPS-stimulated microglia.
Main Results:
- GP-EE significantly decreased mRNA levels of iNOS, TNF-α, IL-1β, Iba-1, and TLR-4 in LPS-stimulated microglia.
- GP-EE also reduced iNOS protein levels.
- The extract demonstrated a regulatory effect on excessive microglial activation.
Conclusions:
- GP-EE exhibits significant anti-inflammatory properties.
- The extract's ability to modulate microglial activation suggests therapeutic potential for neuroinflammation-related neurodegenerative diseases.
- GP-EE is a promising natural extract for functional foods and chemopreventive applications.
