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Updated: Apr 19, 2026
Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus
Published on: March 28, 2025
Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue
Sima Rostami1, Shams Shariat Torbaghan1, Shahriar Dabiri1
1Medical Laboratory of Hazrat Ali Hospital, Alborz University of Medical Sciences, Karaj, Iran; Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran; Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Abstract:
Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, presents an important medical and veterinary problem globally, including that in Iran. Different genotypes of E. granulosus have been reported from human isolates worldwide. This study identifies the genotype of the parasite responsible for human hydatidosis in three provinces of Iran using formalin-fixed paraffin-embedded tissue samples. In this study, 200 formalin-fixed paraffin-embedded tissue samples from human CE cases were collected from Alborz, Tehran, and Kerman provinces. Polymerase chain reaction amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 gene were performed for genetic characterization of the samples. Phylogenetic analysis of the isolates from this study and reference sequences of different genotypes was done using a maximum likelihood method. In total, 54.4%, 0.8%, 1%, and 40.8% of the samples were identified as the G1, G2, G3, and G6 genotypes, respectively. The findings of the current study confirm the G1 genotype (sheep strain) to be the most prevalent genotype involved in human CE cases in Iran and indicates the high prevalence of the G6 genotype with a high infectivity for humans. Furthermore, this study illustrates the first documented human CE case in Iran infected with the G2 genotype.
