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Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
Pentoxifylline modulation of plasma membrane functions in human polymorphonuclear leukocytes
W L Hand1, M L Butera, N L King-Thompson
1Veterans Administration Medical Center (Atlanta), Decatur, Georgia 30033.
Infection and Immunity
|November 1, 1989
Summary
Pentoxifylline modulates human neutrophil functions, inhibiting microbial particle ingestion and superoxide generation while affecting nucleoside and clindamycin uptake. Its mechanism acts beyond membrane receptors, suggesting therapeutic potential for inflammatory conditions.
Area of Science:
- Immunology
- Pharmacology
- Cell Biology
Background:
- Pentoxifylline is known to affect mammalian cell membrane function, particularly in leukocytes.
- Its protective role in inflammation and infection models is linked to phagocyte function, but the precise mechanism remains unclear.
Purpose of the Study:
- To investigate the effects of pentoxifylline on human polymorphonuclear neutrophil membrane-associated activities.
- To explore the underlying mechanisms of pentoxifylline's influence on neutrophil function.
Main Methods:
- Assessed pentoxifylline's impact on microbial particle ingestion by neutrophils.
- Measured superoxide generation and nucleoside/clindamycin uptake under various stimulation conditions.
- Compared pentoxifylline's effects with known nucleoside transport inhibitors.
Main Results:
- Pentoxifylline inhibited microbial particle ingestion and superoxide generation in stimulated neutrophils.
- It decreased adenosine uptake but increased clindamycin uptake in zymosan-stimulated neutrophils.
- Results indicated pentoxifylline does not bind to membrane nucleoside transport receptors, acting downstream of receptor activation.
Conclusions:
- Pentoxifylline significantly modulates stimulated leukocyte membrane-associated responses.
- Its mechanism of action involves the activation signal chain beyond membrane receptors.
- Pentoxifylline shows potential for managing inflammatory diseases.
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