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Related Concept Videos

Transcription01:10

Transcription

160.7K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
160.7K
Responses to Salt Stress02:02

Responses to Salt Stress

15.2K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
15.2K
Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

29.0K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
29.0K
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

32.5K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
32.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

4.4K
4.4K

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Temporal transcriptome changes induced by methyl jasmonate in Salvia sclarea.

Da Cheng Hao1, Shi Lin Chen2, Anne Osbourn3

  • 1Biotechnology Institute, School of Environment, Dalian Jiaotong University, Dalian 116028, China.

Gene
|December 24, 2014
PubMed
Summary

Methyl jasmonate (MeJA) treatment significantly alters gene expression in Salvia sclarea, particularly genes involved in secondary metabolite production. These transcriptomic changes precede the actual increase in plant metabolites, offering insights into plant defense mechanisms.

Keywords:
De novo assemblyGrowthMetabolismMethyl jasmonateSalvia sclareaTranscriptome sequencing

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Area of Science:

  • Plant Molecular Biology
  • Plant Biochemistry
  • Genomics and Transcriptomics

Background:

  • Salvia sclarea (clary sage) is a valuable medicinal and aromatic plant producing phenylpropanoids and terpenoids.
  • Methyl jasmonate (MeJA) is a known plant stress elicitor, but its precise role in enhancing secondary metabolite production in S. sclarea is not fully understood.
  • Understanding MeJA's impact on gene expression is crucial for optimizing the production of valuable plant compounds.

Purpose of the Study:

  • To perform a genome-wide analysis of temporal gene expression in S. sclarea leaves and roots following MeJA treatment.
  • To identify differentially expressed genes (DEGs) and understand their role in secondary metabolite biosynthesis and plant defense.
  • To elucidate the molecular mechanisms underlying MeJA-induced transcriptome reprogramming in S. sclarea.

Main Methods:

  • Genome-wide transcriptome analysis using Illumina RNA-Seq on S. sclarea at 0, 10, and 26 hours post-MeJA treatment.
  • De novo assembly of isogenes and mapping of sequencing reads to identify differentially expressed Unigenes.
  • Quantitative Real-Time PCR (qRT-PCR) to validate RNA-Seq data and extend expression analysis to 73 hours.
  • Chromatographic analysis of secondary metabolites at various time points after MeJA treatment.

Main Results:

  • MeJA treatment induced significant temporal changes in gene expression, with thousands of Unigenes showing differential expression.
  • Upregulation of genes involved in phenylpropanoid biosynthesis, transcription factors, cytochrome P450s, glycosyltransferases, methyltransferases, and transporters was observed.
  • Transcriptomic changes preceded the MeJA-induced accumulation of secondary metabolites, indicating a complex regulatory response.

Conclusions:

  • MeJA elicits a complex and temporally regulated reprogramming of the S. sclarea transcriptome.
  • The study provides a comprehensive understanding of the molecular basis for MeJA-induced secondary metabolism.
  • Results are valuable for characterizing secondary metabolism genes and for breeding S. sclarea varieties with enhanced chemotypes.