Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cancers Originate from Somatic Mutations in a Single Cell

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...

Cancers Originate from Somatic Mutations in a Single Cell

Cancers Originate from Somatic Mutations in a Single Cell

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[DNA informs on ancient partner choice].

Medecine sciences : M/S·2026

Same author

[TimeVaults provide recordings of past cellular activity].

Medecine sciences : M/S·2026

Same author

[In vivo CAR-Ts, a significant breakthrough].

Medecine sciences : M/S·2026

Same author

[House of cards?]

Medecine sciences : M/S·2026

Same author

[Towards gene therapy for Hutington's disease].

Medecine sciences : M/S·2025

Same author

[DNA reveals the living world].

Medecine sciences : M/S·2025

Search research articles

Related Experiment Video

Updated: Apr 19, 2026

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

[Single-cell sequencing and tumour heterogeneity].

Bertrand Jordan¹

¹UMR 7268 ADÉS, Aix-Marseille Université/EFS/CNRS, Espace éthique méditerranéen, hôpital d'adultes la Timone, 264, rue Saint-Pierre, 13385 Marseille Cedex 05, France - CoReBio PACA, case 901, parc scientifique de Luminy, 13288 Marseille Cedex 09, France.

Medecine Sciences : M/S

|December 25, 2014

Summary

Single-cell sequencing reveals extensive genetic diversity within breast tumors before treatment. This tumor heterogeneity can lead to resistance against targeted therapies, impacting treatment efficacy.

More Related Videos

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Apr 19, 2026

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Oncology
Genomics
Molecular Biology

Context:

Single-cell sequencing technologies are revolutionizing our understanding of tumor biology.
Recent studies focus on the genetic landscape of individual cells within tumors.
Breast tumors are a significant area of research due to their prevalence and complexity.

Purpose:

To document the precise heterogeneity of tumors at the single-cell level.
To analyze the patterns of point mutations within individual tumor cells.
To investigate the implications of genetic diversity for targeted therapy resistance.

Summary:

Single-cell new-generation sequencing precisely documents tumor heterogeneity.
Analysis of breast tumors reveals unique point mutation patterns in each cell.
This extensive genetic diversity exists prior to therapeutic intervention.

Impact:

Identifies a key mechanism for resistance to targeted therapies.
Highlights the challenge of achieving complete tumor eradication due to inherent diversity.
Informs the development of more effective and personalized cancer treatment strategies.