Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evening primrose and its compounds of 1-Oxohederagenin and remangilone C ameliorate bleomycin-induced pulmonary fibrosis by regulating β-catenin signaling.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2024

Same author

Engineered nanovesicles mediated cardiomyocyte survival and neovascularization for the therapy of myocardial infarction.

Colloids and surfaces. B, Biointerfaces·2024

Same author

Outer membrane vesicles from commensal microbes contribute to the sponge Tedania sp. development by regulating the expression level of apoptosis-inducing factor (AIF).

Communications biology·2024

Same author

Dietary supplementation with Macleaya cordata extract alleviates intestinal injury in broiler chickens challenged with lipopolysaccharide by regulating gut microbiota and plasma metabolites.

Frontiers in immunology·2024

Same author

Polyaptamer-Driven Crystallization of Alendronate for Synergistic Osteoporosis Treatment through Osteoclastic Inhibition and Osteogenic Promotion.

ACS nano·2024

Same author

Efficacy of hip abductors exercise training combined with repetitive transcranial magnetic stimulation on knee osteoarthritis: A randomized controlled trial.

Technology and health care : official journal of the European Society for Engineering and Medicine·2024

Search research articles

Related Experiment Video

Updated: Apr 19, 2026

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Published on: July 12, 2012

Microfluidic platform integrated with worm-counting setup for assessing manganese toxicity.

Beibei Zhang¹, Yinbao Li², Qidi He¹

¹School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou, Guangdong 510006, China.

Biomicrofluidics

|December 25, 2014

Summary

This summary is machine-generated.

A novel microfluidic system with automated worm responders quantifies manganese toxicity in C. elegans. This system assesses neurotoxicity and oxidative stress, revealing dose-dependent mobility defects and testing antioxidant protective effects.

More Related Videos

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Published on: May 2, 2018

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

Published on: May 2, 2013

Related Experiment Videos

Last Updated: Apr 19, 2026

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Published on: July 12, 2012

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Published on: May 2, 2018

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

Published on: May 2, 2013

Area of Science:

Environmental toxicology
Microfluidics
Neuroscience

Background:

Manganese exposure poses environmental risks.
Assessing manganese toxicity in model organisms is crucial.
Existing methods for toxicity assessment can be labor-intensive.

Purpose of the Study:

To develop and validate a microfluidic system for evaluating environmental manganese toxicity.
To quantitatively assess manganese-induced dopaminergic neurotoxicity and oxidative stress in C. elegans.
To investigate the protective effects of natural antioxidants against manganese toxicity.

Main Methods:

A microfluidic device with automated worm responders utilizing electric impedance sensing for label-free, non-invasive worm counting.
A radial concentration gradient generator (CGG) for automated, simultaneous generation of chemical concentration gradients.
Quantitative assessment of dopaminergic neurotoxicity using green fluorescence protein (GFP)-tagged neurons and oxidative stress via fluorescence intensity in C. elegans strains.
Evaluation of worm survival, stroke frequency, and mobility defects.

Main Results:

The microfluidic system successfully quantified manganese-induced dopaminergic neurotoxicity and oxidative stress in C. elegans.
Dose- and time-dependent mobility defects, including reduced survival and increased stroke frequency, were observed in manganese-exposed worms.
The system demonstrated the potential of natural antioxidants to mitigate manganese toxicity.

Conclusions:

The integrated microfluidic system offers a high-throughput, automated platform for environmental toxicity assessment.
This technology enables precise, quantitative evaluation of neurotoxicity and oxidative stress.
The findings highlight the utility of the microsystem for screening protective compounds against environmental toxins.