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Methylated DNA Immunoprecipitation

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis.

Arpita De¹, Wouter Sparreboom¹, Albert van den Berg¹

¹BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, University of Twente , Enschede 7522NH, The Netherlands.

Biomicrofluidics

|December 25, 2014

Summary

This summary is machine-generated.

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This study introduces a rapid microfluidic system for capturing ultra-low concentrations of hyper-methylated DNA (hm-DNA). The lab-on-a-chip platform efficiently enriches hm-DNA from biological samples for cancer research.

Area of Science:

Biotechnology
Molecular Biology
Cancer Research

Background:

Hyper-methylated DNA (hm-DNA) promoter regions of tumor suppressor genes are crucial for cancer investigation.
Detecting hm-DNA in biological samples like stool and urine is challenging due to its ultra-low concentrations, necessitating enrichment and amplification.

Purpose of the Study:

To develop a rapid microfluidic solid-phase extraction (μSPE) system for capturing and eluting low concentrations of hm-DNA.
To enable efficient enrichment of hm-DNA using a passive lab-on-a-chip platform.

Main Methods:

A microfluidic solid-phase extraction (μSPE) system utilizing a protein-DNA capture surface was developed.
Assay steps were characterized using real-time surface plasmon resonance (SPR) biosensing and fluorescence spectroscopy.

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Last Updated: Apr 19, 2026

Methylated DNA Immunoprecipitation

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Main Results:

The μSPE system successfully captured and eluted hm-DNA at concentrations as low as ≤1 ng/ml.
The capture/elution process was completed in under 5 minutes, achieving 71% efficiency with a 25 μl elution volume and 92% efficiency with a 100 μl elution volume.

Conclusions:

The developed microfluidic μSPE system offers a rapid and efficient method for enriching ultra-low concentrations of hm-DNA.
This technology has potential applications in sensitive cancer diagnostics and biomarker discovery from various biological samples.