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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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TSLP expression: analysis with a ZsGreen TSLP reporter mouse.

Cedric Dewas1, Xi Chen1, Tetsuya Honda2

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892;

Journal of Immunology (Baltimore, Md. : 1950)
|December 26, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed a reporter mouse to track thymic stromal lymphopoietin (TSLP) expression. This tool revealed that TNF-α and IL-4/IL-13 activate keratinocytes to produce TSLP, potentially amplifying allergic inflammation.

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Area of Science:

  • Immunology
  • Allergy Research
  • Molecular Biology

Background:

  • Thymic stromal lymphopoietin (TSLP) is a key cytokine in allergic inflammation.
  • Epithelial cells are a primary source of TSLP, targeting dendritic cells and CD4 T cells.
  • Understanding TSLP's regulation is crucial for developing allergy treatments.

Purpose of the Study:

  • To develop a reporter mouse model for visualizing TSLP expression in vivo.
  • To identify specific inducers of TSLP production in epithelial cells.
  • To investigate the potential role of TSLP in positive feedback loops during allergic responses.

Main Methods:

  • Development of a TSLP-ZsGreen (ZsG) reporter mouse using bacterial artificial chromosome recombineering.
  • Analysis of ZsG expression as a surrogate for TSLP expression in various cell types.
  • Investigating the effects of TNF-α, IL-4/IL-13, and T-cell activation on keratinocyte TSLP production.

Main Results:

  • The TSLP-ZsG reporter mouse accurately reflects TSLP expression, particularly in keratinocytes and thymic epithelial cells.
  • Tumor necrosis factor-alpha (TNF-α) and interleukins 4/13 (IL-4/IL-13) strongly induce TSLP expression in keratinocytes.
  • Activated Th2 and Th1 cells can induce TSLP expression in keratinocytes.

Conclusions:

  • TSLP-ZsG reporter mice provide a valuable tool for studying TSLP biology.
  • Keratinocyte TSLP production is regulated by inflammatory signals like TNF-α and Th2 cytokines.
  • TSLP may contribute to sustained allergic inflammation through a positive feedback loop involving Th2 cells.