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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
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Characterizing phenotype with tracer based metabolomics.

Wai Nang P Lee1

  • 1Department of Pediatrics, Harbor-UCLA Medical Center, 1124 W. Carson Street, Torrance, CA 90502 USA.

Metabolomics : Official Journal of the Metabolomic Society
|December 26, 2014
PubMed
Summary
This summary is machine-generated.

Tracer-based metabolomics analyzes cellular phenotypes by tracking stable isotopes. This method reconstructs metabolic pathways to understand gene-phenotype correlations and cellular responses to environmental changes.

Keywords:
constraint based modelingphenotypic phase plane analysisreaction networktracer based metabolomics

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Area of Science:

  • Systems Biology
  • Metabolomics
  • Genomics

Background:

  • Understanding gene function, nutrient interactions, and phenotype origins is crucial in the post-genomic era.
  • Metabolomics quantifies small molecules to describe cellular phenotypes.
  • Genotype-phenotype correlation can be approached via metabolic map reconstruction.

Purpose of the Study:

  • To review tracer-based metabolomic methods for constructing phenotypic phase plane plots.
  • To discuss the functional implications of phenotypic phase plane analysis.
  • To provide examples of phenotypic changes in response to biological and environmental perturbations.

Main Methods:

  • Utilizing reaction network analysis to predict pathway utilization for phenotypic characterization.
  • Employing stable isotope tracers in tracer-based metabolomics for reaction network analysis.
  • Analyzing the redistribution of isotope tracers among metabolic intermediates to identify key pathways.

Main Results:

  • Tracer-based metabolomics enables the identification of a finite set of utilized pathways.
  • Pathway utilization is characteristic of cellular phenotypic behavior.
  • Phenotypic phase plane plots can be constructed to visualize cellular phenotypes.

Conclusions:

  • Tracer-based metabolomics is a powerful experimental approach for reaction network analysis.
  • Phenotypic phase plane analysis offers insights into functional implications of cellular states.
  • This methodology can characterize phenotypic changes due to differentiation, pathway inhibition, or nutrient environment alterations.