Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

4.5K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.5K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

4.5K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
4.5K
Hematopoiesis01:21

Hematopoiesis

10.2K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
10.2K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

4.2K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
4.2K
Lineage Commitment01:21

Lineage Commitment

4.6K
Commitment is the  process whereby stem cells:
4.6K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

12.4K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Overexpression of PPENK reduces myocardial ischemia reperfusion injury by promoting mitophagy in rats].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2017
Same author

Laser-Induced Breakdown Spectroscopy for Rapid Discrimination of Heavy-Metal-Contaminated Seafood Tegillarca granosa.

Sensors (Basel, Switzerland)·2017
Same author

Selective Acceleration of Crystal Growth of Indomethacin Polymorphs by Low-Concentration Poly(ethylene oxide).

Molecular pharmaceutics·2017
Same author

Operationalization of diagnostic criteria of DSM-5 somatic symptom disorders.

BMC psychiatry·2017
Same author

[Prospects of the Application of Photoacoustic Technique in Microcirculation Imaging Research on the Acupoints Sensitization].

Zhen ci yan jiu = Acupuncture research·2017
Same author

Psychological and somatic distress in Chinese outpatients at general hospitals: a cross-sectional study.

Annals of general psychiatry·2017
Same journal

Invaders taking over-Mollusc faunal change in volcanic barrier lakes of the Albertine Rift biodiversity hotspot.

PloS one·2026
Same journal

AI-driven molecular diversification and ligand-based optimization of macitentan derivatives targeting VEGFR1 and endothelin signaling pathways.

PloS one·2026
Same journal

Performance patterns and records in the world aquatics masters championships: Where do the most frequently represented nations among the top-ten masters swimmers come from?

PloS one·2026
Same journal

Modeling diurnal Temperature-Rainfall relationships under multicollinearity using PLS-SEM: A case study of Ghana.

PloS one·2026
Same journal

Organizational culture, social capital, and emergency capacity in primary healthcare institutions: A cross-sectional structural equation modeling study comparing ordinary and older communities.

PloS one·2026
Same journal

Impact of kidney function on the metabolome in the general population.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Apr 19, 2026

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

9.0K

ARAP3 functions in hematopoietic stem cells.

Yiwen Song1, Jing Jiang2, Sonja Vermeren3

  • 1Department of Pediatrics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, United States of America; Division of Hematology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America.

Plos One
|December 27, 2014
PubMed
Summary
This summary is machine-generated.

Arap3 (ARAP3) is crucial for embryonic blood vessel development. While its absence impairs hematopoietic stem cells (HSCs) in adults, this study finds ARAP3 is not cell-autonomously required for HSC function.

More Related Videos

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

10.3K
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

12.0K

Related Experiment Videos

Last Updated: Apr 19, 2026

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

9.0K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

10.3K
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

12.0K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Hematology

Background:

  • ARAP3 (Arapidopsis thaliana Ras-associated protein 3) is a GTPase-activating protein that regulates Arf6 and RhoA small GTPases.
  • ARAP3 deficiency in mice leads to embryonic lethality due to defective angiogenesis.
  • Mutant mice (Arap3KI/KI) with impaired ARAP3 activation exhibit similar angiogenic defects, with some surviving to adulthood.

Purpose of the Study:

  • To investigate the cell-autonomous and non-cell-autonomous roles of ARAP3 in hematopoiesis and hematopoietic stem cell (HSC) function.
  • To determine if ARAP3 deficiency impacts HSC reconstitution and self-renewal capabilities.
  • To explore the impact of ARAP3 deletion in specific bone marrow niche cells on HSC homeostasis.

Main Methods:

  • Conditional deletion of Arap3 in hematopoietic cells using Vav1-Cre.
  • Conditional deletion of Arap3 in osteoblasts and mesenchymal stromal cells using Prx1-Cre.
  • Conditional deletion of Arap3 in endothelial cells using vascular endothelial cadherin (VEC/Cdh5)-driven Cre.
  • Hematopoietic stem cell transplantation assays.
  • In vitro proliferation and differentiation assays.

Main Results:

  • ARAP3 is not cell-autonomously required for HSC proliferation, differentiation, multi-lineage reconstitution, or self-renewal.
  • Deletion of ARAP3 in osteoblasts and mesenchymal stromal cells did not affect hematopoietic development or HSC homeostasis.
  • Conditional deletion of Arap3 in endothelial cells resulted in embryonic lethality, but HSCs from surviving adults were largely normal and showed no functional deficits upon transplantation.

Conclusions:

  • ARAP3 does not play a cell-autonomous role in hematopoietic stem and progenitor cells.
  • While ARAP3 is critical for embryonic vascular development, its absence in endothelial cells has minimal impact on HSCs in adult bone marrow.
  • The study highlights a critical role for ARAP3 in embryonic angiogenesis, distinct from its function in adult HSC maintenance.