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Filaggrin expression in cutaneous and mucosal human papillomavirus induced lesions
J Viac1, I Guérin-Reverchon, M C Chignol
1INSERM U 209, CNRS DO 601, Hôpital E. Herriot, Lyon, France.
Abstract:
A series of 32 human papillomavirus induced lesions derived from epidermis and mucosa was studied for the modulation of filaggrin-profilaggrin (F-PF) expression according to the degree of virus infection as compared to normal skin and mucosa biopsies. This investigation was carried out on frozen sections using indirect immunofluorescence for filaggrin detection and group specific viral antigen and by in situ hybridization with biotinylated probes for viral DNA detection and typing. The 9 cutaneous warts showed an increase of F-PF expression in upper layer cells as compared to normal epidermis, which could be related to the high production of virus (viral antigen and HPV types 1 or 2). The 5 condyloma acuminata displayed also an enhanced expression of these components which was located in several upper layers but virus infection was confirmed in 2 of them with HPV types 6, 11 or 16. The 6 laryngeal papillomas exhibited a granular reactivity pattern for F-PF in suprabasal cell layers with an increase in the upper layers; viral antigen was found in 4 cases and HPV DNA types 6, 11 or 16 were detected in 4 specimens. Conversely among 12 cervical intraepithelial neoplasia, F-PF was expressed only in very superficial layers in few cases, without any correlation with the DNA detection (6, 11 or 16, 18). Taken together these data are suggestive of an intense expression of F-PF in benign lesions which can replicate the virus and a discrete or an absent expression of these components in premalignant or malignant lesions.
Insights
Human papillomavirus (HPV) infection impacts filaggrin-profilaggrin (F-PF) expression. Benign HPV lesions show increased F-PF, while premalignant and malignant lesions exhibit reduced or absent F-PF expression.
Area of Science:
- Dermatology
- Virology
- Molecular Biology
Background:
- Filaggrin-profilaggrin (F-PF) is a key structural protein in the epidermis.
- Human papillomavirus (HPV) infections can alter cellular differentiation and protein expression.
- Understanding F-PF modulation in HPV lesions is crucial for diagnosis and prognosis.
Purpose of the Study:
- To investigate the expression patterns of filaggrin-profilaggrin (F-PF) in various HPV-induced lesions.
- To correlate F-PF expression levels with the degree of HPV infection and lesion type.
- To differentiate F-PF expression between benign, premalignant, and malignant HPV-related conditions.
Main Methods:
- Analysis of 32 HPV-induced lesions (cutaneous warts, condyloma acuminata, laryngeal papillomas, cervical intraepithelial neoplasia) and normal biopsies.
- Indirect immunofluorescence for filaggrin and viral antigen detection.
- In situ hybridization for HPV DNA detection, typing, and quantification.
Main Results:
- Cutaneous warts and condyloma acuminata showed increased F-PF expression, correlating with high HPV viral load (HPV types 1, 2, 6, 11, 16).
- Laryngeal papillomas exhibited altered F-PF patterns with detectable viral antigen and HPV DNA (types 6, 11, 16).
- Cervical intraepithelial neoplasia displayed minimal F-PF expression, with no clear correlation to HPV DNA detection (types 6, 11, 16, 18).
Conclusions:
- Intense F-PF expression is characteristic of benign HPV lesions capable of viral replication.
- Discrete or absent F-PF expression is associated with premalignant and malignant HPV lesions.
- F-PF expression serves as a potential biomarker for distinguishing benign from pre-malignant/malignant HPV-related conditions.