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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
[Effect of fluoride on the viability and apoptosis of ameloblasts in vitro]
Lin Ma1, Ying Zhang, Ming Zhong
1Department of Preventive Dentistry, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China.E-mail:jrma6143@sina.com.
Purpose:
To evaluate the effect of fluoride on viability of rat ameloblasts in vitro.
Methods:
The ameloblasts of rat was exposed to different concentrations of NaF (0, 0.4, 0.8, 1.6, 3.2, 6.4 mmol/L) for 24, 48 and 72 hours. CCK-8 assays were performed to measure the cells proliferation; The morphology of apoptosis was observed by Hoechst 33258 staining and the rate of apoptosis was determined by flow cytometry. The data was analyzed using SPSS 13.0 software package.
Results:
(1)The proliferation of ameloblasts was increased when concentrations of NaF between 0.4 mmol/L and 0.8 mmol/L, whereas inhibited at 1.6 mmol/L NaF and above. The effects were in time-dependent manner.(2)Cells in the 1.6 mmol/L NaF groups showed unclear karyorrhexis and apoptotic cell morphology. The effects were in concentration-dependent manner.
Conclusions:
(1)Fluoride has two-phase effects to ameloblasts: At low doses, it promoted cell proliferation while at high doses it had negative effects. (2)1.6 mmol/L NaF could induce apoptosis of ameloblasts.
