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Updated: Jul 22, 2026
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
[The effects of ADAM28 AS-ODN on biological function of human gingival fibroblasts]
Zheng Zhao1, Li Wei, Lu-Jia Li
1Department of Stomatology, the 401 Hospital of Chinese PLA. Qingdao 266071, Shandong Province, China.E-mail:zhaozheng170@163.com.
Purpose:
To study the effects of a disintegrin and metalloproteinase 28 (ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs), and analyze the possible mechanism.
Methods:
Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry (FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.0 software package.
Results:
In ADAM28 AS-ODN group, the proliferation activity of HGFs decreased significantly. Cell percentage in S phase in AS-ODN group was notably lower than that of S-ODN and untransfected groups, and cell percentage in G2+M phase was remarkably lower than that of untransfected group. Cell proliferation index (PI=S+G2M) in AS-ODN group was significantly lower than that of the other two groups. There was a significant difference between the groups. In AS-ODN group, alkaline phosphatase (ALP) secretion activity and percentage of apoptotic cell notably increased.
Conclusions:
ADAM28 AS-ODN could inhibit HGFs proliferation significantly and influence the changes of cell cycle, promote HGFs differentiation and induce HGFs apoptosis remarkably.
