Related Experiment Video
Updated: Apr 19, 2026

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
Published on: November 21, 2014
Interaction of recombinant octameric hemoglobin with endothelial cells
Caroline Gaucher1, Élisa Domingues-Hamdi2, Christine Prin-Mathieu3
1Université de Lorraine, UMR CNRS 7561, Groupe Ingénierie Cellulaire et Tissulaire, Faculté de médecine, 5450 Vandoeuvre-lès-Nancy, France; Université de Lorraine, CITHEFOR EA 3452, Faculté de Pharmacie, BP 80403, 54001 Nancy Cedex, France.
Abstract:
Hemoglobin-based oxygen carriers (HBOCs) may generate oxidative stress, vasoconstriction and inflammation. To reduce these undesirable vasoactive properties, we increased hemoglobin (Hb) molecular size by genetic engineering with octameric Hb, recombinant (r) HbβG83C. We investigate the potential side effects of rHbβG83C on endothelial cells. The rHbβG83C has no impact on cell viability, and induces a huge repression of endothelial nitric oxide synthase gene transcription, a marker of vasomotion. No induction of Intermolecular-Adhesion Molecule 1 and E-selectin (inflammatory markers) transcription was seen. In the presence of rHbβG83C, the transcription of heme oxygenase-1 (oxidative stress marker) is weakly increased compared to the two other HBOCs (references) or Voluven (control). This genetically engineered octameric Hb, based on a human Hb βG83C mutant, leads to little impact at the level of endothelial cell inflammatory response and thus appears as an interesting molecule for HBOC development.
More Related Videos
Related Concept Videos
Hemoglobin
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
Oxygen Transport in the Blood
Regulation of Angiogenesis and Blood Supply
Multiple Allele Traits
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Cooperative Allosteric Transitions

