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Is hydroxyethyl starch necessary for sedimentation of bone marrow?
Margriet J Dijkstra-Tiekstra1, Airies C Setroikromo1, Marcha Kraan1
1Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands.
Abstract:
Hydroxyethyl starch (HES) is used to separate hematopoietic progenitor cells after bone marrow (BM) collection from red blood cells. The aims were to study alternatives for HAES-steril (200 kDa; not available anymore) and to optimize the sedimentation process. Using WBC-enriched product (10 × 10(9) WBC/L), instead of BM, sedimentation at 10% hematocrit using final 0.6 or 0.39% Voluven (130 kDa) or without HES appeared to be good alternatives for 0.6% HAES-steril. MNC recovery >80% and RBC depletion >90% was reached. Optimal sedimentation was reached using 110-140 mL volume. Centrifugation appeared not suitable for sedimentation. Additional testing with BM might be necessary to confirm these results.
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