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In-vitro Mutagenesis

In-vitro Mutagenesis

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Related Experiment Video

Updated: Apr 19, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

IAP gene deletion and conditional knockout models.

John Silke¹, David L Vaux¹

¹The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, Victoria 3050, Australia.

Seminars in Cell & Developmental Biology

|December 30, 2014

Summary

This summary is machine-generated.

Gene deletion studies reveal inhibitor of apoptosis (IAP) proteins

Keywords:

BIR Birc Gene deletion IAP NAIP Survivin

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Area of Science:

Cellular biology
Immunology
Genetics

Background:

Inhibitor of Apoptosis Proteins (IAPs) play crucial roles in cellular processes.
Understanding their functions is vital for immunity and cell division.

Purpose of the Study:

To elucidate the distinct and shared functions of IAP proteins.
To investigate the roles of cIAP1, cIAP2, and XIAP in innate immunity.
To differentiate the function of Survivin from other IAPs.

Main Methods:

Gene deletion studies in mice.
Analysis of IAP mutant phenotypes.
Comparative analysis of IAP homologs across species.

Main Results:

cIAP1, cIAP2, and XIAP regulate innate immune responses to pathogens without causing autoimmunity.
Survivin and its homologs are not involved in innate immunity or apoptosis regulation.
Survivin functions in chromosome segregation during mitosis with INCENP, aurora kinase B, and Borealin.

Conclusions:

IAP proteins have specialized roles in immunity and cell division.
Survivin's function is distinct from IAPs involved in immune regulation.