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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: Apr 19, 2026

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

The human pancreas proteome defined by transcriptomics and antibody-based profiling.

Angelika Danielsson¹, Fredrik Pontén¹, Linn Fagerberg²

¹Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

|December 30, 2014

Summary

Researchers identified key genes and proteins highly expressed in the human pancreas using RNA sequencing and protein profiling. This reveals the pancreas

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Last Updated: Apr 19, 2026

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

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Published on: July 18, 2019

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

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Published on: March 23, 2020

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Published on: May 4, 2012

Area of Science:

Molecular Biology
Human Physiology
Genomics

Background:

The pancreas has dual exocrine and endocrine functions, crucial for digestion and blood glucose regulation.
Understanding the pancreas' molecular composition is vital for studying its diseases and functions.

Purpose of the Study:

To identify genes and proteins with elevated expression in the human pancreas.
To map these molecular constituents to specific cell types within the pancreas.

Main Methods:

Genome-wide RNA sequencing of the human transcriptome.
Immunohistochemistry-based protein profiling.
Analysis of whole pancreas and isolated endocrine islets.

Main Results:

Identified 146 genes with elevated expression in the pancreas, with 47 deemed pancreas-enriched.
Discovered an additional 42 genes highly expressed in isolated endocrine islets.
Found that a small fraction of highly expressed genes (0.7%) constitute 68% of pancreatic mRNA, indicating specialization in secreted protein production.
Identified novel proteins in both endocrine and exocrine pancreatic cells.

Conclusions:

Provides a comprehensive global analysis of the pancreas transcriptome and proteome.
Offers a valuable resource for future research into pancreatic cell biology, diseases, and treatments.