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Updated: Apr 19, 2026

Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay
Published on: July 26, 2022
Lipoic-based TRPA1/TRPV1 antagonist to treat orofacial pain
Roberta Gualdani, Stefania Ceruti1, Giulia Magni1,2
1∥Lab of Molecular and Cell. Pharm. Purinergic Transmission, Department of Pharmacological Biomol. Sciences (DiSFeB), University of Milan, 20122 Milan, Italy.
Abstract:
Inflammation of the trigeminal nerve is considered one of the most painful conditions known to humankind. The diagnosis is often difficult; moreover, safe and effective pharmacological treatments are lacking. A new molecule, ADM_12, formed by a lipoic and omotaurine residues covalently linked, is here reported. In vitro and in vivo tests showed that ADM_12 is a very attractive original compound presenting (i) a remarkable safety profile; (ii) a high binding constant versus TRPA1; (iii) an intriguing behavior versus TRPV1; and (iv) the ability to significantly and persistently reduce mechanical facial allodynia in rats. Noteworthy, by testing ADM_12, we shed light on the unprecedented involvement of TRPA1 and TRPV1 channels in orofacial pain.
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