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A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120
A Hemming1, L Lundberg, S Olofsson
1Department of Clinical Virology, University of Göteborg, Sweden.
Archives of Virology
|January 1, 1989
Abstract:
By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M 13 for further mutagenesis and is also easy to reclone.