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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Related Experiment Video

Updated: Apr 19, 2026

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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Immunohistochemistry in dermatopathology.

Tammie Ferringer1

  • 1From the Departments of Dermatology and Laboratory Medicine, Geisinger Medical Center, Danville, Pennsylvania.

Archives of Pathology & Laboratory Medicine
|December 31, 2014
PubMed
Summary

Immunohistochemistry is a valuable tool in dermatopathology, aiding diagnosis but requiring contextual interpretation. While not always specific or sensitive, it helps resolve diagnostic challenges.

Area of Science:

  • Dermatopathology
  • Immunohistochemistry
  • Diagnostic techniques

Background:

  • Immunohistochemistry (IHC) is a crucial tool in dermatopathology.
  • IHC requires careful interpretation within the clinical context.
  • It is not a standalone diagnostic test.

Purpose of the Study:

  • To review the current applications of immunohistochemistry in dermatopathology.
  • To discuss the limitations of immunohistochemistry in dermatopathology.

Main Methods:

  • Literature review of English-language publications.
  • Search period: 1980 to 2014.

Main Results:

  • Immunohistochemistry is frequently used in dermatopathology.

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  • Its specificity and sensitivity can be limited.
  • It aids in resolving diagnostic dilemmas.

    • Conclusions:

    • Immunohistochemistry is an important adjunctive technique in dermatopathology.
    • It assists in complex diagnostic cases.
    • Contextual interpretation is essential for accurate IHC use.