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Updated: Apr 19, 2026

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
Published on: March 1, 2014
In vitro development of canine somatic cell nuclear transfer embryos in different culture media
Dong-Hoon Kim1, Jin-Gu No, Mi-Kyung Choi
1Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.kimdhhj@korea.kr.
Abstract:
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
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