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Updated: Apr 19, 2026
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
Published on: June 7, 2016
Diminished expression of MiR-15a is an independent prognostic marker for breast cancer cases
Yoshiaki Shinden1, Sayuri Akiyoshi2, Hiroki Ueo3
1Department of Surgery, Kyushu University Beppu Hospital, Beppu, Oita, Japan Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Kagoshima, Japan.
Background/Aim:
MiR-15a targets Cyclin E1 (CCNE1), which regulates the cell cycle and promotes cell proliferation and progression. Herein, we investigated the clinicopathological significance of miR-15a as a prognostic marker in breast cancer (BC) cases.
Materials And Methods:
We collected primary tumor samples of 230 BC cases, including 68 triple-negative cases. The expression levels of miR-15a in primary tumors were measured by qRT-PCR assay.
Results:
Low expression of miR-15a in primary tumors was significantly correlated with shorter disease-free survival (p=0.0012) and overall survival (p=0.005) compared to the high miR-15a expression in triple-negative BC cases. Multivariate analysis indicated that low miR-15a expression was an independent prognostic factor for overall survival [RR=2.56(1.03-7.18), p=0.04].
Conclusion:
MiR-15a expression levels could be a promising biological and prognostic marker for overall survival especially in triple-negative BC cases.
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