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Dendrofalconerol A suppresses migrating cancer cells via EMT and integrin proteins
Premkamol Pengpaeng1, Boonchoo Sritularak2, Pithi Chanvorachote3
1Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Background/Aim:
Enhanced cell motility is a hallmark of highly metastatic cancer cells. The anti-migratory activity of Dendrofalconerol A (DF-A), a pure bis(bibenzyls) isolated from the stem of Dendrobium falconeri (Orchidaceae) is reported in the present study.
Materials And Methods:
Cytotoxicity effects of DF-A on H460 lung cancer cells was determined by the MTT assay. We also investigated the mechanism of DF-A-mediated EMT and integrin proteins level by western blotting.
Results:
DF-A at concentrations of 0.5-5 μM significantly reduced the protein level of migrating cells in a dose-dependent manner. The expression of migration-related integrins, including integrin β1 and integrin α4 was significantly reduced in response to DF-A treatment. Also, DF-A was shown to suppress epithelial to mesenchymal transition (EMT), as indicated by cadherin switch from N- to E-cadherin and decrease of Snail, Slug and vimentin.
Conclusion:
This study revealed the potential of DF-A, an anti-metastasis agent and the underlying mechanism in this in vitro assay with H460 cells, which leads to the development of a novel anti-metastatic agent.
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