Related Experiment Video
Updated: Apr 19, 2026

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
Published on: April 12, 2019
Interfering effect of bilirubin on the determination of alkaline phosphatase
Zhong Wang1, Hongyan Guo1, Ying Wang1
1Beijing You'an Hospital Clinical Laboratory Center, Capital Medical University Beijing 100069.
Objective:
This study was designed to evaluate whether the high concentration of bilirubin is able to interfere the determination of alkaline phosphatase (ALP).
Methods:
Clinical tests evaluating the interfering substance of bilirubin of various concentrations on the determination of ALP were conducted based upon the Clinical and Laboratory Standards Institute (CLSI) document EP7-A2, the most recent guideline on interference testing approved in 2005.
Results:
Paired t-test comparing different doses of bilirubin revealed that the concentration of 1 000 μmmol/L bilirubin negatively interfered the determination of ALP levels. The experiment designed with five different concentrations of bilirubin showed that bilirubin can exert negative interference on the measurement of ALP in a linear pattern.
Conclusion:
High concentration of bilirubin can cause false measurement of ALP levels, probably interfering with the clinical prognosis of liver diseases.
More Related Videos
06:33Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
Published on: July 5, 2021
09:32Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
Related Concept Videos
Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test
Jaundice
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow