Supercritical fluid chromatography and scale up study

The influence of process parameters on supercritical fluid chromatography (SFC) was investigated together with scaling-up to the preparative scale. Within this scope, separation of model compounds (caffeine and theophylline), dependencies on pressures, temperatures, types and concentrations of the modifiers, and types of stationary phases, were examined separately. Experiments were performed on analytical scale and on pilot preparative scale SFC apparatus. Bare silica and silica 2-ethylpyridine were used as stationary phases, with CO(2)/methanol or CO(2)/ethanol at elevated pressures and temperatures as mobile phase. Observations obtained on the analytical scale were used for scaling-up to preparative scale. The aim of this study is evaluating the influences of process parameters when separating model compounds using SFC, and s practical demonstration of the scaling-up method of SFC along with the operation of a pilot preparative to production scale SFC apparatus, which is one of the bigger similar apparatuses worldwide.