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Platelet-activating factor causes goblet cell depletion in the conjunctiva
D F Woodward1, C S Spada, A L Nieves
1Allergan, Inc., Discovery Research, Irvine, CA 92715.
European Journal of Pharmacology
|September 1, 1989
Summary
Platelet-activating factor (PAF) directly depletes conjunctival goblet cells and increases microvascular permeability. Other mediators like LTD4, LTE4, and histamine have different effects on goblet cells and leukocytes.
Area of Science:
- Ophthalmology
- Immunology
- Cell Biology
Background:
- Conjunctival goblet cells are crucial for ocular surface health.
- Platelet-activating factor (PAF) is implicated in inflammatory responses.
Purpose of the Study:
- To investigate the effects of PAF and related compounds on conjunctival goblet cells and microvascular permeability.
- To compare PAF's effects with those of LTD4, LTE4, and histamine.
Main Methods:
- Administration of PAF, LTD4, LTE4, histamine, and lyso-PAF to conjunctival tissue.
- Assessment of goblet cell population changes.
- Evaluation of leukocyte infiltration and microvascular permeability.
- Use of PAF antagonist CV-6209 to block PAF receptors.
Main Results:
- PAF caused dose-dependent goblet cell depletion and increased microvascular permeability.
- LTD4 and LTE4 induced eosinophil infiltration but not goblet cell loss.
- Histamine led to goblet cell depletion secondary to eosinophil degranulation.
- Lyso-PAF showed effects at high doses, blocked by CV-6209, suggesting PAF receptor involvement.
Conclusions:
- PAF has direct detrimental effects on conjunctival goblet cells and ocular surface barrier function.
- PAF-induced effects are distinct from those of cysteinyl leukotrienes and histamine.
- PAF antagonists may be beneficial in treating conditions involving PAF-mediated ocular inflammation.