Related Experiment Video
Updated: Aug 12, 2026

VisualEyes: A Modular Software System for Oculomotor Experimentation
Published on: March 25, 2011
Vectors permitting visual monitoring of simple transposition events
M Kozlowski1, D Wilkinson, N Nash
1Allelix Biopharmaceuticals, Mississauga, Ont., Canada.
Abstract:
The construction and use of two novel transposon(Tn)-delivery vectors is described. These vectors carry Inc.W or Inc.N broad-host-range transfer functions cloned next to the narrow-host-range replicon of pBR329. The host specificities of pSLX10 and pSLX23 both complement and extend the host specificities of existing Tn delivery vectors. Plasmids pSLX10 and pSLX23 were shown to transfer at high frequency in intergeneric matings. The lux genes which are present on each vector permit the visual monitoring of transconjugants which have retained a Tn element, but are devoid of plasmid molecules. pSLX10 and pLSX23 were efficiently used to generate a range of auxotrophic mutants in various strains of Pseudomonas as well as to clone genes from Serratia liquefaciens. These vectors may have general applicability to identify and clone genes in a wide range of Gram-negative bacteria.
Related Concept Videos
Vector Algebra: Graphical Method
We use the laws of geometry to construct resultant vectors, followed by trigonometry to find vector magnitudes and directions. For a geometric construction of the sum of two vectors in a plane, we follow the parallelogram rule. Suppose two vectors are at arbitrary positions. Translate either one of...
Position and Displacement Vectors
Further, several important kinds of...
Position Vectors
For instance, we want to locate a point P(x, y, z) relative to the origin of coordinates O. In that case, we can define a position...
Vector Transformation in Rotating Coordinate Systems
Position and Displacement Vectors
Further, several important kinds of...
Vectors

