Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vermamoeba vermiformis resides in water-based heater-cooler units and can enhance Mycobacterium chimaera survival after chlorine exposure.

The Journal of hospital infection·2022
Same author

Genetic and epigenetic determinants of reactivation of Mecp2 and the inactive X chromosome in neural stem cells.

Stem cell reports·2022
Same author

Simple urine storage protocol for extracellular vesicle proteomics compatible with at-home self-sampling.

Scientific reports·2021
Same author

Acanthamoeba castellanii interferes with adequate chlorine disinfection of multidrug-resistant Pseudomonas aeruginosa.

The Journal of hospital infection·2020
Same author

Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens.

Optics letters·2020
Same author

Signal reversal in Kelvin-probe force microscopy.

The Review of scientific instruments·2019

Related Experiment Video

Updated: Apr 19, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.4K

Automated spherical aberration correction in scanning confocal microscopy.

H W Yoo1, M E van Royen2, W A van Cappellen2

  • 1Delft Center for Systems and Control, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands.

The Review of Scientific Instruments
|January 3, 2015
PubMed
Summary

Automated adjustment of microscope coverslip correction collars compensates for refractive index mismatches. This improves image resolution in scanning confocal microscopy, reducing spherical aberrations for clearer biological specimen imaging.

More Related Videos

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
10:28

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

894
Clarifying and Imaging Candida albicans Biofilms
11:09

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

14.6K

Related Experiment Videos

Last Updated: Apr 19, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.4K
High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
10:28

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

894
Clarifying and Imaging Candida albicans Biofilms
11:09

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

14.6K

Area of Science:

  • Microscopy and Imaging
  • Optical Physics
  • Biotechnology

Background:

  • Spherical aberrations in microscopy arise from refractive index mismatches between immersion media and glass coverslips, particularly with high numerical aperture objectives.
  • These aberrations degrade image quality and resolution, hindering accurate observation of biological specimens.

Purpose of the Study:

  • To demonstrate an automated method for adjusting the coverslip correction collar in scanning confocal microscopy.
  • To compensate for spherical aberrations caused by coverslip thickness variations and refractive index mismatches.

Main Methods:

  • Utilized a motorized coverslip correction collar for automated adjustment.
  • Employed xz image scans, image processing, and correction quality evaluation to estimate refractive index mismatch.
  • Developed and evaluated coarse-fine Gaussian fitting algorithms for rapid, low-photodamage correction and accurate mismatch estimation.

Main Results:

  • Successfully demonstrated automated adjustment of the coverslip correction collar to compensate for spherical aberrations.
  • Validated the effectiveness of the proposed method across various coverslip types and biological specimens.
  • Achieved optimized resolution in confocal microscopy images through automated aberration correction.

Conclusions:

  • Automated coverslip correction collar adjustment is an effective strategy to mitigate spherical aberrations in scanning confocal microscopy.
  • The proposed method, utilizing Gaussian fitting algorithms, offers fast and accurate compensation, minimizing photodamage.
  • This technique significantly enhances imaging performance and resolution for diverse biological samples.