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Polyprotein processing of Theiler's murine encephalomyelitis virus.

R P Roos1, W P Kong, B L Semler

  • 1Department of Neurology, University of Chicago Medical Center, Illinois 60637.

Journal of Virology
|December 1, 1989
PubMed
Summary

Theiler's murine encephalomyelitis virus polyprotein processing was studied using in vitro translation. Protein 3C performed most cleavages, with a second proteinase active at a specific junction, impacting viral gene expression.

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Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Theiler's murine encephalomyelitis viruses (TMEV) are significant pathogens.
  • Understanding viral polyprotein processing is crucial for TMEV pathogenesis.
  • Previous studies on picornaviruses highlight the role of specific proteinases.

Purpose of the Study:

  • To investigate the polyprotein processing mechanisms of Theiler's murine encephalomyelitis viruses (TMEV).
  • To identify the specific proteinases responsible for TMEV polyprotein cleavage.
  • To explore potential alternative translation initiation sites in TMEV DA strain.

Main Methods:

  • In vitro translation reactions using transcripts from infectious full-length TMEV DA strain cDNA clones.
  • Modification of cDNA templates via restriction endonuclease linearization, linker insertion, or deletion mutations.

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  • Analysis of protein products to identify cleavage sites and active proteinases.

    • Main Results:

    • Protein 3C was identified as the primary proteinase responsible for most polyprotein cleavages in TMEV.
    • A second, distinct proteinase activity was observed at the L-P1-2A junction.
    • An alternative translation product, potentially from an out-of-frame initiation site, was detected in DA strain transcripts.

    Conclusions:

    • Protein 3C plays a central role in TMEV polyprotein processing, consistent with other picornaviruses.
    • The identified second proteinase activity may contribute to specific cleavage events.
    • Alternative translation initiation could influence TMEV gene expression and potentially disease pathogenesis.