Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Influence of several experimental parameters on inter and intra specific mating process efficiency.

V Sambri1, M A Lovett

  • 1Institute of Microbiology, University of Bologna, S. Orsola Hospital, Italy.

Microbiologica
|October 1, 1989
PubMed
Summary

This study optimized bacterial mating for efficient genetic transformation. Media composition and plasmid size significantly impact the success rate of transferring genetic material.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-cholera Vibrio spp. invasive infections in the summer following May 2023 flood disaster in Romagna, Italy: a case series.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2024
Same author

Cu-based thin rolled foils: relationship among alloy composition, micromechanical and antiviral properties against SARS-CoV-2.

Heliyon·2024
Same author

Containment of carbapenem-resistant Enterobacterales colonisations and infections: Results from an integrated infection control intervention in a large hospital trust of northern Italy.

American journal of infection control·2023
Same author

Subgingival periodontal pathogens in Down syndrome children without periodontal breakdown. A case-control study on deciduous teeth.

European journal of paediatric dentistry·2022
Same author

How to manage children if a second wave of COVID-19 occurs.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2020
Same author

COVID-19: its impact on dental schools in Italy, clinical problems in endodontic therapy and general considerations.

International endodontic journal·2020

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bacterial conjugation, a natural gene transfer process, is widely used in molecular biology.
  • Optimizing mating efficiency is crucial for genetic manipulation and strain engineering.

Purpose of the Study:

  • To investigate the impact of experimental parameters on bacterial mating efficiency.
  • To identify key factors influencing the success of plasmid transfer during bacterial conjugation.

Main Methods:

  • Utilized a standard bacterial mating technique.
  • Systematically varied parameters including temperature, growth media, and plasmid size.
  • Quantified transformation efficiency by counting recipient cells successfully transformed.

Related Experiment Videos

Main Results:

  • Growth media composition was identified as the most critical factor affecting mating efficiency.
  • Plasmid size also plays a significant role, interacting with media conditions.
  • Temperature had a less pronounced effect compared to media and plasmid size.

Conclusions:

  • Bacterial conjugation efficiency is highly dependent on the choice of growth media.
  • The interplay between media and plasmid size is essential for successful genetic transfer.
  • This research provides insights for optimizing bacterial transformation protocols.