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Related Experiment Videos

A small nuclear RNA, U5, can transform cells in vitro.

K Hamada1, T Kumazaki, K Mizuno

  • 1Department of Pathology, Hiroshima University, Japan.

Molecular and Cellular Biology
|October 1, 1989
PubMed
Summary

Small nuclear RNA U5, identified in lymphoma cells, can transform cells and induce chromosome damage. This RNA

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Area of Science:

  • Molecular biology
  • Cell biology
  • Oncology

Background:

  • Low-molecular-weight RNA with transforming potential was detected in lymphoma cells.
  • The RNA was isolated from chemically induced lymphoma cells after mink lung cell transformation.

Purpose of the Study:

  • To identify and characterize the RNA with transforming potential.
  • To investigate the mechanism of U5-induced cell transformation and its effects on oncogenes and chromosomes.

Main Methods:

  • RNA isolation and sequencing using the direct chemical method.
  • Quantitative transformation assays using 3Y1 rat fibroblastic cells.
  • Analysis of c-myc oncogene amplification and chromosome aberrations in U5-transformed cells.
  • RNase H treatment with complementary oligonucleotides to inactivate RNA function.

Main Results:

  • The transforming RNA was identified as small nuclear RNA U5.
  • U5 transfection induced cell transformation with a latency of 3-4 weeks.
  • U5-transformed cells frequently exhibited c-myc oncogene amplification.
  • U5 acted as a clastogen, inducing chromosome aberrations.
  • Both transforming and clastogenic activities of U5 were inactivated by RNase H treatment.

Conclusions:

  • Small nuclear RNA U5 possesses transforming and clastogenic potential.
  • U5 may contribute to cell transformation by inducing c-myc amplification and chromosome instability.
  • Further research is needed to elucidate the precise mechanism of U5-induced cell transformation.

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