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Updated: Apr 19, 2026

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
Published on: November 16, 2011
Hypoglycaemic effects of functional tri-peptides from silk in differentiated adipocytes and streptozotocin-induced
Bok Kyung Han1, Hyun Jung Lee2, Hyun-Sun Lee3
1BK Bio Co. Ltd, Sungnam, 462-819, Republic of Korea.
Background:
In this study, the tri-peptides Gly-Glu-Tyr (GEY) and Gly-Tyr-Gly (GYG), identified previously as active compounds from the silk peptide E5K6, significantly stimulated basal and insulin-mediated glucose uptake by 3T3-L1 fibroblasts in a dose-dependent manner.
Results:
Synthetic GEY and GYG peptides at a concentration of 500 µmol L(-1) significantly increased glucose transporter type 4 expression by 157% and 239%, respectively. Differentiation of 3T3-L1 cells into adipocytes leads to accumulation of intracellular fat droplets, and GEY and GYG at a concentration of 250 µmol L(-1) suppressed this effect by 72% and 75%, respectively. GYG improved glucose tolerance in steptozotocin (STZ)-induced diabetic mice in a dose-dependent manner.
Conclusion:
These results suggest that GYG isolated from E5K6 has anti-diabetic potential and silk waste products containing bioactive peptides could be used to the developments of treatments to lower blood glucose.
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