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Modified humoral leukocyte adherence inhibition test for detection of lung cancer
T Kubasova1, M Horváth, I Szarvas
1Frédéric Joliot-Curie, National Research Institute for Radiobiology and Radiohygiene, Tétényi Hospital, Budapest, Hungary.
Abstract:
The freshly separated indicator cells (suspension of leukocytes) used in humoral leukocyte adherence inhibition test were labeled either with 14C-amino acid mixture or 3H-concanavalin A (3H-ConA). Instead of counting the adherent cells, the amount of 'adherent' radioactivity was measured by a liquid scintillation spectrometer. By the modified method, sera from 25 lung-carcinoma-bearing patients as well as serum samples from 21 healthy persons were examined in the presence of crude antigens prepared from 'normal' lung tissue or lung tumors of various histologic types. Although the results demonstrated high specificity and reproducibility of both methods, the binding of 3H-ConA to the surface of adherent cells is more expressed and assures higher levels of radioactivity.