Surface plasmon resonance (SPR) biosensors in pharmaceutical analysis

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Pharmaceutical Sciences

Background:

• Surface Plasmon Resonance (SPR) is a powerful optical biosensing technology.
• SPR has demonstrated significant utility across various stages of pharmaceutical development.
• Existing literature often compartmentalizes SPR applications in drug discovery versus quality control.

Purpose of the Study:

• To comprehensively review the applications of SPR biosensors in drug discovery.
• To highlight the role of SPR in the quality analysis of pharmaceutical compounds.
• To present a unified view of SPR's contribution to both drug development and quality assurance.

Main Methods:

• Review of SPR assay formats and experimental considerations.
• Analysis of SPR applications in pharmacokinetic profiling and ADMET studies.
• Examination of SPR in high-throughput screening and fragment-based drug screening, focusing on small molecule detection.
• Inclusion of localized SPR and SPR imaging techniques.

Main Results:

• SPR facilitates efficient pharmacokinetic drug profiling and ADMET assessment.
• SPR is effective for high-throughput and fragment-based screening of drug candidates.
• SPR biosensors enable sensitive detection of small drug molecules and analysis of pharmaceutical quality.

Conclusions:

• SPR biosensors offer a robust platform for advancing drug discovery pipelines.
• SPR technology is crucial for ensuring the quality and safety of pharmaceutical products.
• This review consolidates SPR's impact across the entire pharmaceutical lifecycle.