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Determination of thailandepsin B in rat plasma with a LC-MS/MS method
Zhongzhe Cheng1, Lian Ding, Yang Zhang
1Tongji School of Pharmacy, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan, Hubei 430030, China.
Background:
Thailandepsin B (TDP-B) is a potent histone deacetylase inhibitor under development. A reliable bioanalytical method for the quantification of TDP-B in plasma samples is required.
Results:
The stabilizer mixture containing hydrochloric acid, formic acid and dichlorvos was applied to stabilize TDP-B in matrix samples. The method validation was conducted over the curve range of 1.00 to 1000 ng/ml. The intrabatch and interbatch precision and accuracy of the quality control samples showed ≤10.9% relative standard deviation and -6.7% to 15.0% relative error. Moreover, a possible metabolite M of TDP-B was tentatively characterized.
Conclusion:
A reliable LC-MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.
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