Development and characterization of a diamond-insulated graphitic multi electrode array realized with ion beam...

Area of Science:

• Neuroscience
• Materials Science
• Biosensor Technology

Background:

• Detecting quantal exocytic events from neurons is challenging.
• Diamond offers biocompatibility, transparency, and chemical inertness for biosensor development.
• Conductive diamond can be fabricated via ion implantation and annealing.

Purpose of the Study:

• To develop a novel diamond-based biosensor for detecting catecholamine release.
• To create a 16-channel multi-electrode array (MEA) for cell culture applications.

Main Methods:

• Fabrication of a 16-channel MEA using ion implantation on a single-crystal diamond.
• Focused 1.2 MeV He+ beam scanning to create damaged sub-superficial structures.
• Annealing to convert damaged regions into a conductive graphitic phase.
• 3D masking to create surface-contacting electrodes.
• Cyclic voltammetry and amperometry for biosensor characterization.

Main Results:

• Successful fabrication of a 16-channel diamond MEA.
• Demonstrated conversion of damaged diamond regions to conductive graphitic phase.
• Characterization using adrenaline solutions showed sensitivity to catecholamine concentrations.
• The biosensor is suitable for in vitro detection of catecholamine release.

Conclusions:

• A novel diamond-based biosensor has been successfully developed.
• The biosensor is effective for in vitro detection of catecholamine release.
• This technology holds promise for advancing neuroscience research.