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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

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Updated: Apr 19, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide.

Jason D Buenrostro^1,2, Beijing Wu¹, Howard Y Chang²

¹Department of Genetics, Stanford University School of Medicine, Stanford, California.

Current Protocols in Molecular Biology

|January 7, 2015

Summary

Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) maps genome-wide chromatin accessibility. This method uses Tn5 transposase to identify accessible DNA regions for downstream analyses like transcription factor binding and nucleosome positioning.

Keywords:

ATAC-seq chromatin accessibility transposase

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Last Updated: Apr 19, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

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Published on: June 30, 2022

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Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

Chromatin accessibility is crucial for gene regulation.
Existing methods for genome-wide chromatin accessibility mapping have limitations.
Understanding nucleosome positioning and transcription factor binding requires precise genomic tools.

Purpose of the Study:

To describe the Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) method.
To highlight ATAC-seq as a tool for mapping genome-wide chromatin accessibility.
To present ATAC-seq as an efficient alternative to other established techniques.

Main Methods:

Utilizes hyperactive Tn5 transposase to probe DNA accessibility.
Tn5 transposase inserts sequencing adapters into accessible chromatin regions.
High-throughput sequencing of adapter-inserted DNA fragments.

Main Results:

Enables genome-wide mapping of accessible chromatin regions.
Facilitates inference of transcription-factor binding sites.
Allows for the determination of nucleosome positions within accessible regions.

Conclusions:

ATAC-seq is a fast and sensitive method for assaying chromatin accessibility.
The technique provides a comprehensive view of the accessible genome.
ATAC-seq offers advantages over DNase-seq and MNase-seq for specific applications.