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Updated: Aug 19, 2026

07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
Published on: September 3, 2013
[A non-enzymatic method of preparing isolated hepatocytes: assessment of their morpho-functional activity]
Abstract:
A method for isolating hepatocytes using the two-step liver perfusion by chelate-containing solutions is described. The hepatocytes obtained are highly inactive for the trypan blue staining test, (not less than 90%), ATP content, the rate of endogenic respiration and its stimulation by succinate, a degree of xenobiotic oxidation.

